Lumiprobe 蛋白質標記試劑盒 Protein labeling kits

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Lumiprobe protein labeling kits contain pre-aliquoted reactive dye, all necessary auxiliary components and buffers, and desalting spin columns to achieve efficient, reproducible labeling of antibodies, and other proteins.

The labeled protein is purified by means of gel filtration on gel filled spin columns that are enclosed. This protocol ensures complete removal of the unbound dye, and excellent recovery of the labeled product. The protein is eluted in PBS buffer, ready for the use in most downstream applications.

The protocol is quick, effortless, and easy to follow.

Lumiprobe 探針蛋白標記試劑盒包含預先引用的活性染料、所有必需的輔助成分和緩沖器，以及脫鹽自旋柱，以實現抗體和其他蛋白質的高效、可復制的標記。

標記蛋白是通過凝膠過濾純化的凝膠填充自旋柱是封閉的。該協議確保完全去除未結合染料，并出色地恢復標記產品。該蛋白在PBS緩沖液中被洗脫，可以在大多數下游應用中使用。

該試劑盒具有快速，毫不費力，易于遵循等優點。

Lumiprobe Cy7.5-N-羥基琥珀酰亞胺酯

Lumiprobe PEP azide熒光染料

Phenylethynylpyrene（PEP）熒光素屬于多環芳烴類標記物，有較高靈敏度，是對微環境進行標記的試劑。類似于Pyrene，PEP dye很容易形成激發物。然而，PEP熒光多為紅移。PEP可以用作微環境探針，基于激發態標記實驗。

Phenylethynylpyrene (PEP) fluorophore is a polyaromatic hydrocarbon label with high sensitivity to microenvironment. Similarly to pyrene, PEP dye readily forms excimers. However, PEP fluorescence is more red-shifted

PEP can be used as microenvironment probe, and as a label for assays based on excimer formation.

This reagent contains triethyleneglycol linker to facilitate dissolution of non-polar PEP dye in organo-aqueous labeling reaction mixtures. With this azide, and Click Chemistry, it is easy to turn any molecule bearing alkyne into PEP-labeled probe.

General properties

Spectral properties

Lumiprobe TAMRA azide, 5- isomer

TAMRA (TMR, tetramethylrhodamine) azide，有固體和10mM溶于DMSO兩種形式。用于點擊化學的標記試劑。是純化的5-isomer。TAMRA通常用于FAM熒光團的熒光共振能量轉移（FRET）受體。

TAMRA (TMR, tetramethylrhodamine) azide, solid compound, and 10 mM solution in DMSO, labeling reagent for Click Chemistry. Pure 5-isomer.

TAMRA is often used as FRET acceptor for FAM fluorophore.

Can replace Alexa Fluor 555, DyLight 549.

General properties

Spectral properties

供應Sulfo-Cyanine5 ?azide上海金畔生物

用于點擊化學的水溶Cyanine5 dye azide。高亮度耐光的紅色染料。在水中的溶解度較高。由于其高溶解度和親水性，該染料在單純的水環境中即可對生物分子進行標記。
Sulfo-Cyanine5是Cy5?的類似物，是一種非常流行的熒光染料，能與很多熒光設備相兼容，比如成像儀，讀板機，顯微鏡等。
該試劑是1:1混合的crystallosolvate和methanol。但不會對熒光團性能和應用有任何影響。

Water-soluble Cyanine5 dye azide for Click chemistry. Bright and photostable dye with red fluorescence. The reagent has high water solubility. Due to high solubility and hydrophilicity, the dye can be used for labeling biomolecules in native, purely aqueous conditions.

This reagent, as opposed to competing products, is supplied as an alkaline metal salt and not triethylammonium salt. Therefore, it is a powder which is easy to handle and dispense.

Sulfo-Cyanine5 is an analog of Cy5?, a very popular fluorophore, therefore this reagent is compatible to a wide range of standard fluorescent instrumentation such as imagers, plate readers, and microscopes.

General properties

Spectral properties

Lumiprobe Sulfo-Cyanine3 azide

Sulfo-Cyanine3 azide是用于點擊化學的水溶疊氮染料。其激發和發射波長與Cy?熒光團相同。
由于這種染料的高亮度，耐光性，再加上在水中高溶解度的特點，該染料成為點擊化學中水相標記各種分子的理想選擇。
在水相中不需有機共溶劑即可進行點擊反應（Click reaction）。該染料是為標記敏感的分子如蛋白甚至完整的生物體而設計。
該試劑是1:1混合的crystallosolvate和methanol。但不會對熒光團性能和應用有任何影響。

Sulfo-Cyanine3 azide is a water-soluble dye azide for Click Chemistry. Absorbance and emission of the dye are identical to Cy3? fluorophore.

Brightness and photostability of fluorophore, together with high water solubility, makes this reagent an ideal choice for Click chemistry labeling of various molecules in aqueous phase.

Click reaction can be carried out in pure water without need to use organic co-solvent. The reagent is designed for the labeling of sensitive molecules such as proteins, and even intact biological objects.

General properties

Spectral properties