日本JaICA老化制御研究所代理

上海金畔生物代理日本JaICA產品，歡迎新老客戶訪問日本JaICA官網或者咨詢我們獲取更多相關產品信息。

日本JaICA代理

日本老化制御研究所代理

日本老化制御研究所自成立以來就對抗衰老進行研究，它是在有助于健康促進的理念下創建的。結果，此研究所在開發DNA氧化損傷測試試劑盒方面取得了很大的成功，并在該領域處于了領先地位。
本研究所現在銷售許多氧化應激相關產品，這些產品可應用于醫學，藥學，生化學，食品科學等。它還利用其獨特的技術開展檢測服務。隨著社會老齡化的發展，預防醫學與抗衰老引起了越來越多的關注，在這些領域中，我們研究所將會通過開發和提供研究產品來助于營造一個健康，長壽的社會。

產品類型：
1.???????? DNA氧化檢測產品；
2.???????? 蛋白氧化檢測產品；
3.???????? 脂肪氧化檢測產品；
4.???????? 抗氧化檢測產品。

特性：
1.???????? 高專一性、高靈敏性；
2.???????? 不與RNA損傷產生交叉反應；
3.???????? 易于操作、簡單快速；
4.???????? 無需昂貴的設備和樣品預處理。

The Japan Institute for the Control of Aging has conducted research into control of aging since its foundation under the doctrine of contributing health promotion. As a result, the institute succeeded in developing a DNA oxidative damage measuring kit, thus taking the lead for this field.
The institute now sells many products related to oxidative stress, and such products are utilized in the fields of medicine, pharmacy, biochemistry, food sciences, etc. The institute also performs assay services utilizing its unique technologies. Preventive medicine and anti-aging are receiving increased attention with the advancement of aging society. In these areas, our Institute will contribute to realizing a healthy, long-lived society by developing and supplying research products.

Type of Products：

1. DNA oxidation;
2. Protein oxidation;
3. Lipid oxidation;
4. Antioxidant assay.

Features：

l??????????High specificity & High sensitivity;

l??????????No cross-reactivity with RNA damage;

l??????????Easy operation & Speedy;

l??????????There is?no need for expensive equipment and sample pretreatment.

日本老化制御研究所

日本JaICA Test kit for Potential Anti Oxidant (PAO)

日本老化制御研究所?Jaica代理

上海金畔生物作為日本 JaICA 老化中國代理商，歡迎新老客戶訪問日本 JaICA 老化官網或者咨詢我們獲取更多日本 JaICA 老化產品線價格說明書等信息。

Suitable for detection of total antioxidant capacity in serum and food extracts. For research use only.

Antioxidant assay:

Oxidative stress plays on important role in various diseases and aging. The control of oxidative stress is expected to be useful to prevent diseases and aging.Oxidative stress is caused by the imbalance between reactive oxygen species (ROS) and antioxidant defense system. For accurate assessment of oxidative stress, measurement of ROS, oxidative damage and antioxidant activity may be essential. Recently, antioxidants as functional foods which scavenge ROS attract a great deal of attention.

Principle of this assay:

In the PAO assay kit, an easy and convenient method to measure antioxidant capacity is provided. Utilizing the reduction of cupric ion (Cu++ to Cu+), antioxidant capacity of samples can be detected in 5 minutes. Samples are mixed with Cu++ Solution. Cu++ are reduced by antioxidants to form Cu+. Reduced Cu+ react with Chromatic Solution (Bathocuproine) , and can be detected by absorbance at wavelength 480 to 490 nm. Antioxidant capacity can be calculated from the Cu+ formed. PAO can detect not only hydrophilic antioxidants such as Vitamin C, glutathione, but also can detect hydrophobic antioxidants such as Vitamin E. Applicable for assessment of total antioxidants of serum, foods and beverage samples.

Specifications:

Content of this kit: 試劑盒組成

Assay procedure:

References

日本JaICA Test kit for Potential Anti Oxidant (PAO)? ?說明書? 技術資料

日本Japan Institute for the Control of Aging (JaICA)

日本老化制御研究所?Jaica代理

上海金畔生物作為日本 JaICA 老化中國代理商，歡迎新老客戶訪問日本 JaICA 老化官網或者咨詢我們獲取更多日本 JaICA 老化產品線價格說明書等信息。

日本JaICA試劑盒產品目錄

日本老化制御研究所?Jaica代理

上海金畔生物作為日本 JaICA 老化中國代理商，歡迎新老客戶訪問日本 JaICA 老化官網或者咨詢我們獲取更多日本 JaICA 老化產品線價格說明書等信息。

日本 JaICA代理 Anti Di Tyrosine (DT) 單抗

日本老化制御研究所自成立以來就對抗衰老進行研究，它是在有助于健康促進的理念下創建的。結果，此研究所在開發DNA氧化損傷測試試劑盒方面取得了很大的成功，并在該領域處于了領先地位。

本研究所現在銷售許多氧化應激相關產品，這些產品可應用于醫學，藥學，生化學，食品科學等。它還利用其獨特的技術開展檢測服務。隨著社會老齡化的發展，預防醫學與抗衰老引起了越來越多的關注，在這些領域中，我們研究所將會通過開發和提供研究產品來助于營造一個健康，長壽的社會。

產品類型：

DNA氧化檢測產品；

蛋白氧化檢測產品；

脂肪氧化檢測產品；

抗氧化檢測產品。

特性：

高專一性、高靈敏性；

不與RNA損傷產生交叉反應；

易于操作、簡單快速；

無需昂貴的設備和樣品預處理。

上海金畔生物作為日本 JaICA 老化中國代理商，歡迎新老客戶訪問日本 JaICA 老化官網或者咨詢我們獲取更多日本 JaICA 老化產品線價格說明書等信息。

日本老化制御研究所?Jaica代理

日本 JaICA代理 Anti Di Tyrosine (DT) 單抗

產品名稱：?Anti Di Tyrosine (DT) 單抗

英文名稱：?Anti Di Tyrosine (DT) monoclonal antibody

產品貨號：?MDT-020P

產品規格：?20ug

產品產地：?日本

產品商標：?JaICA

日本JaICA 代理Hexanoyl-Lysine adduct(HEL) ELISA Kit

Suitable for assessment of oxidative stress using urine, serum and cultured cells. For research use only.

已酰-賴氨酸（HEL）ELISA試劑盒，適用于尿液，血清和細胞培養樣品氧化應激的評估。
僅供研究使用。

什么是己酰-賴氨酸？

Biomarker for early stage of lipid oxidation

Oxidative damage of lipids caused by reactive oxygen species (ROS) play an important role in some diseases, lesion of cell functions and aging. Aldehydes such as malondi-aldehyde (MDA) and 4-hydroxy-2-nonenal (4-HNE) have been reported as one of the advanced lipid peroxidation products. But recently in the earlier stage of lipid peroxidation, 13-hydroperoxyoctadecanoic acid (13-HPODE) is found to be covalently bound to proteins1). Hexanoyl-Lysine adduct (HEL) is a novel lipid hydroperoxide-modified lysine residues. HEL is formed by oxidative modification by oxidized omega-6 fatty acids such as linoleic acid or arachidonic acid. HEL may be a useful biomarker for initial stage of lipid peroxidation.Monoclonal antibodies and ELISA kit have been developped, and HEL can be detected in oxidatively modified LDL, in human atherosclerotic lesions, human urine and serum. It is also reported that HEL is formed in rat muscle during exercise, and the formation is prohibited by antioxidants such as flavonoids.

由活性氧（ROS）引起的脂質氧化損傷在一些疾病、細胞功能損害和衰老中起重要作用。醛類如丙二醛（MDA）和4-羥基-2-壬烯醛（4-HNE）已被報道為先進的脂質過氧化產物之一。但最近在脂質過氧化的早期階段，發現13-氫過氧十八烷酸（13-HPODE）與蛋白質共價結合。己酰-賴氨酸加合物（HEL）是一種新的脂質氫過氧化物修飾的賴氨酸殘基。HEL是通過氧化的omega-6脂肪酸（例如亞油酸或花生四烯酸）的氧化修飾形成。HEL可能是脂質過氧化初始階段有用的生物標志物?，F在我們已經開發了HEL單克隆抗體和ELISA試劑盒，并且可以在氧化修飾的低密度脂蛋白（LDL）、人動脈粥樣硬化病變、人尿液和血清中檢測HEL。另據報道，大鼠肌肉在運動期間形成HEL，并且通過抗氧化劑（如類黃酮）是禁止形成的。

己酰-賴氨酸（HEL）ELISA試劑盒

JaICA have developed HEL ELISA kit in collaboration with Dr. Toshihiko Osawa (Nagoya University) and Dr. Yoji Kato (Univerisity of Hyogo). This ELISA kit can be applied to urine, serum and cultured cells form human and animal.

JaICA公司與Toshihiko Osawa博士（名古屋大學）和Yoji Kato博士（兵庫大學）合作開發了Hexanoyl-Lys（HEL）ELISA試劑盒。
該ELISA試劑盒可應用于人和動物的尿液、血清和細胞培養。

技術參數
檢測原理：競爭法ELISA (檢測: 450 nm處)；
特異性：特異于N-epsilon-己酰-賴氨酸加合物；
測定范圍：2 – 700 nmol/L；
反應速度：過夜+2小時；
規格：96孔(54 samples in single assay)；
應用：人和動物的尿液、血清和細胞培養物；
儲存：2-8℃（避免冷凍）；
保質期：2年。

Content of this kit

試劑盒組成：

HEL-包被微孔板: 1 塊板(96孔)
一抗 (即用型): 1?瓶
二抗: 1?瓶
二抗緩沖液: 1?瓶
底物溶液 (TMBZ溶液): 1?瓶
底物緩沖液: 1 瓶
洗滌液 (5X): 1 瓶
終止液: 1 瓶
標準液 (6 標準): 各1 瓶
封板膜: 2 片

參考文獻：

1)
Yoji Kato, Yoko Mori, Yuko Makino, Yasujiro Morimitsu, Sadayuki Hiroi, Toshitsugu Ishikawa, Toshihiko Osawa, Formation of N epsilon-(hexanonyl) lysine in protein exposed to lipid hydroperoxide. J Biol Chem 274(29), p20406-20414 (1999)
Identification of HEL, which is lysine adduct of 13-HPODE.
2)
Yoji Kato, Toshihiko Osawa: Detection of lipid hydroperoxide-derived protein modification with polyclonal antibodies. Methods in Molecular Biology, vol. 186, Oxidative stress biomarkers and antioxidant protocols, D Armstrong, Ed., Human Press Inc., NJ, USA, p37-44
Immunohistochemical detection of hexanoyl-lysine adduct.
3)
Kato Y, Miyake Y, Yamamoto K, Shimomura Y, Ochi H, Mori Y, Osawa T, Preparation of a monoclonal antibody to N(epsilon)-(Hexanonyl)lysine: application to the evaluation of protective effects of flavonoid supplementation against exercise-induced oxidative stress in rat skeletal muscle. Biochem Biophys Res Commun 274(2),p389-393(2000)
Development and characterization of anti HEL monoclonal antibody.
4)
Ryo K, Yamada H, Nakagawa Y, Tai Y, Obara K, Inoue H, Mishima K, Saito I, Possible involvement of oxidative stress in salivary gland of patients with Sjogren’s syndrome. Pathobiology 73(5), p252-260 (2006)
HEL can be detected in saliva samples from patients with Sjogren’s syndrome.
5)
Suzuki T, Kazui T, Yamamoto S, Washiyama N, Ohkura K, Ohishi K, Bashar AH, Yamashita K, Terada H, Suzuki K, Akuzawa S, Fujie M, Effect of prophylactically administered edaravone during antegrade cerebral perfusion in a canine model of old cerebral infarction. J Thorac Cardiovasc Surg 133(3),p710-716 (2007)
Application to canine serum samples.
6)
Shimizu K, Ogawa F, Akiyama Y, Muroi E, Yoshizaki A, Iwata Y, Komura K, Bae S, Sato S, Increased Serum Levels of N(epsilon)-(hexanoyl)lysine, A New Marker of Oxidative Stress, in Systemic Sclerosis. J Rheumatol. 2008 Sep 1.
Serum HEL concentration from systemic sclerosis is higher than that from healthy subjects.
7)
Tamura H, Takasaki A, Miwa I, Taniguchi K, Maekawa R, Asada H, Taketani T, Matsuoka A, Yamagata Y, Shimamura K, Morioka H, Ishikawa H, Reiter RJ, Sugino N, Oxidative stress impairs oocyte quality and melatonin protects oocytes from free radical damage and improves fertilization rate. J Pineal Res 44(3),p280-287(2008)
Intrafollicular concentration of HEL was significantly reduced by these antioxidant treatment.
8)
Sakamoto Y, Ishikawa T, Kondo Y, Yamaguchi K, Fujisawa M, The assessment of oxidative stress in infertile patients with varicocele. BJU Int 101(12), p1547-1552 (2008)
Azoospermic and oligospermic patients had a significantly higher HEL concentration in seminal plasma.
9)
Kageyama Y, Takahashi M, Nagafusa T, Torikai E, Nagano A, Etanercept reduces the oxidative stress marker levels in patients with rheumatoid arthritis. Rheumatol Int 28(3),pp245-251(2008)
Urinary HEL level was reduced at 3 and 6 months after the initial treatment with etanercept.
10)
Rummenie VT, Matsumoto Y, Dogru M, Wang Y, Hu Y, Ward SK, Igarashi A, Wakamatsu T, Ibrahim O, Goto E, Luyten G, Inoue H, Saito I, Shimazaki J, Tsubota K, Tear cytokine and ocular surface alterations following brief passive cigarette smoke exposure. Cytokine 43(2),p200-208(2008)
HEL concentration in tear was increased by brief passive exposure to cigarette smoke.
11)
K Sakai, S Kino, A Masuda, M Takeuchi, T Ochi, J Osredkar, B Rejc, K Gersak, N Ramarathnam, Y Kato;
Determination of HEL (Hexanoyl-Lysine Adduct): A Novel Biomarker for Omega-6 PUFA Oxidation.
Lipid Hydroperoxide-Derived Modification of Biomolecules: Subcellular Biochemistry 77,p61-72(2014)
An original paper for HEL ELISA development.

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使用手冊????????質量控制??????? 技術資料