Lumiprobe PEP azide熒光染料
Phenylethynylpyrene（PEP）熒光素屬于多環芳烴類標記物，有較高靈敏度，是對微環境進行標記的試劑。類似于Pyrene，PEP dye很容易形成激發物。然而，PEP熒光多為紅移。PEP可以用作微環境探針，基于激發態標記實驗。
Phenylethynylpyrene (PEP) fluorophore is a polyaromatic hydrocarbon label with high sensitivity to microenvironment. Similarly to pyrene, PEP dye readily forms excimers. However, PEP fluorescence is more red-shifted
PEP can be used as microenvironment probe, and as a label for assays based on excimer formation.
This reagent contains triethyleneglycol linker to facilitate dissolution of non-polar PEP dye in organo-aqueous labeling reaction mixtures. With this azide, and Click Chemistry, it is easy to turn any molecule bearing alkyne into PEP-labeled probe.
General properties
| Appearance: | off white / yellowish solid |
| Molecular weight: | 544.64 |
| Molecular formula: | C34H32N4O3 |
| Solubility: | soluble in dichloromethane, chloroform, moderately soluble in DMSO, DMF, acetonitrile |
| Quality control: | NMR 1H (95%) and 13C, TLC, functional testing |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Excitation maximum, nm: | 293; 362; 384 |
| Emission maximum, nm: | 389 |
Spectral properties
| 訂購貨號 | 產品名稱及規格 |
| A4530 | PEP azide, 1 mg |
| B4530 | PEP azide, 5 mg |
| C4530 | PEP azide, 10 mg |
| D4530 | PEP azide, 25 mg |
| E4530 | PEP azide, 50 mg |